Calcium chloride in Transformation
The exact reason/mechanism involved in the use of CaCl2 too increase cell permeability is not well-known. Its function was determined through empirical means / trial and error. =)
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In Groove with Life
***** A site for my classes because life goes beyond prescribed text and there is so much to share and to learn. And of course, since lessons are often punctuated with hysterical laughter and incoherent mind flow, here is a venue to organise my thought processes.
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3 comments:
hey sir! this could be of interest!
http://www.dnai.org/text/mediashowcase/index2.html?id=1009
it's even animated man! it claims that the Ca2+ ions interact with negative charges of the hydrophilic heads of phospholipids, creating an electrostatically neutral environment for DNA to enter via adhesion zones.
however,
http://www.genome.ou.edu/protocol_book/protocol_adxF.html
seems to disagree with this hypothesis.
" The bacterial cell membrane is permeable to chloride ions, but is non-permeable to calcium ions. As the chloride ions enter the cell, water molecules accompany the charged particle. This influx of water causes the cells to swell and is necessary for the uptake of DNA. The exact mechanism of this uptake is unknown."
maybe it's a combination of both? you know, through heating the adhesion zones are widened sufficiently for Ca2+ to enter the cell along with the DNA?
cheerios,
mryeo
http://www.bookrags.com/Transformation_(genetics)
"The calcium ions precipitate the DNA on the surface of the cell, where the DNA is forced into the recipient."
not a lot about CaCl2 but quite interesting to read anyway about the new method of transformation!
okay that's about as much time as i have for random reading. time to start mugging actual bio stuff for promos. blah.
cheerios,
mryeo
There are indeed many ways to introduce plasmids these days. :)
Heat shock is also very common. In fact the concentration of CaCl2 also be a variable :)
cool.
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