Someone asked:
we have used lac and trp operon in synthesizing human growth factor/ insulin and anti-thrombin. Does it matter which operon is used?
Ans: Nope. Don't worry about it because the examples are given because that was just what people had worked with in making those protein. By now, I am sure things have moved on and they may be using other operons. =)
Can we use a RNA primer?
Does it matter if we have a RNA:DNA hybrid if we just want to check the sequence?
Ans:It doesnt really matter just that there is no reason to have such a hybrid. Why use RNA when DNA primers can also be readily synthesized?
Also, will we have end-replication problem if we use RNA primers?
Ans: No, because there will not be any DNA polymerase coming along behind the primer to remove it and fill the space with DNA. so The primers will flank the sequence/gene of interest.
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In Groove with Life
***** A site for my classes because life goes beyond prescribed text and there is so much to share and to learn. And of course, since lessons are often punctuated with hysterical laughter and incoherent mind flow, here is a venue to organise my thought processes.
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