Titillating Question of Mid_eek

In line with the SATS mugging, I shall use more adjectives hahaa


Point Mutations (FYI):

1. Missense mutation: substitution of a nucleotide → change in amino acid e.g. sickle cell anemia
2. Nonsense mutation: substition of a nucleotide → form a stop codon → cause premature termination of polypeptide elongation.
3. Silent mutation: due to a degenerate code, substitution of a nucleotide that does not cause change in the resultant amino acid
4. Frameshift mutation: due to insertion/deletion of a nucleotide.

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Effect of pH in electrophoresis

For non-chemistry students:

Why pH is important in electrophoresis because amino acids have their own isoelectric point. At a particular pH, an amino acid may be neutral (zwitterion) while others will be positive or negative. Thus application of charge will separate them.

http://chimge.unil.ch/En/ph/1ph81.htm (see the example)

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Why twins have different fingerprints

Since I always forget half of what I read, better you read it :P
http://forensic-evidence.com/site/ID/ID_Twins.html


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Since we use PolyT oligomer as a primer for mRNA (template) to eventually form ds cDNA, would that also imply that the resultant ds cDNA has polyA and polyT sequences within it?

Interesting perspective and the answer is YES.